PRKN and neuroblastoma: To ascertain that TKI increases parkin ubiquitination, B35 rat neuroblastoma cells (Gift of Dr. Cathy Conant) were transfected with 3 μg parkin cDNA and treated with either 10 μM nilotinib or 1 μM bosutinib for 24 hrs after transfection and the proteasome was inhibited with 20 μM MG132 for 6 hrs.