Here, in order to find molecular targets of RNase A, we performed an analysis of genome-wide profiles of miRNAs in the tumour and serum of mice bearing Lewis lung carcinoma (LLC), after treatment with RNase A by high-throughput Sequencing by Oligonucleotide Ligation and Detection (SOLiDTM) sequencing technology. The gene discussed is RNASE1; the disease is Carcinoma, Lewis Lung.