However, from the 12 cell lines analysed, there was no correct sized band for Nectin-1, only MDA-MB-436 and BT-474KC breast cancer cell lines expressed the correct sized band for Nectin-2, only BT-482 breast cancer cell lines strongly showed the correct band for Nectin-3 (with very weak bands for MDA-MB-436 and MDA-MB-231 cells) and Nectin-4 was not correctly expressed in any cell lines. This evidence concerns the gene NECTIN2 and breast cancer.