To determine whether HoxD10 could transcriptionally regulate the expression of IGFBP3 in gastric cancer cells, we observed the expression level of IGFBP3 after stably introducing HoxD10 gene into BGC823 and SGC7901 cells, or transiently knocking down of HoxD10 in HGC27 cells. This evidence concerns the gene HOXD10 and gastric cancer.