Real-time PCR carried out with the same primer concentrations and numbers of amplification cycles as conventional PCR revealed a single sharp peak in all samples using the Cμ primer set whereas samples from dogs with B and T cell lymphoma had distinct large peaks with the corresponding primer sets IgH major, minor and TCRγ (Figure 1). The gene discussed is TRGC1; the disease is T-cell non-Hodgkin lymphoma.