To further investigate the effect of exogenous cell surface GRP78 on the invasive potential of hepatocellular carcinoma cells, we constructed GRP78 KDEL motif deleted mutant (ΔKDEL) and transfected SMMC7721 cells with the ΔKDEL mutant and the cells stably overexpressing GRP78 on the cell surface were selected by G418 (400 μg/mL). The gene discussed is HSPA5; the disease is hepatocellular carcinoma.