The tumor sections in the control group revealed 75% ± 12.6 of positive staining of p-STAT3 while only around 12.4 ± 8.6 and 5.4 ± 4.5% of cells were stained positive for p-STAT3 in the treatment groups injected with 5 mg/kg and 10 mg/kg of berberine respectively (p <0.05). The gene discussed is STAT3; the disease is neoplasm.