However, when expressed in certain cancer cell lines (e.g., SKBr3 breast cancer cells), the reporter exhibits an enhanced plasma membrane localization [28], even under serum-starved conditions, that is likely due to constitutive activation of signaling pathways (e.g., EGFR activation or Her2 overexpression) that lead to activation of PI3K in the absence of exogenous stimuli. The gene discussed is ERBB2; the disease is cancer.