CDH5 and neoplasm: To semiquantitatively test for differential Ad vector-mediated host organ versus tumor expression, we immunoblotted extracts from both KO and SC tumors as well as liver, in hCAR:Rag2−/− mice injected with 5.0×1010 vp of either the Ad5ROBO4 (n = 4 mice) or Ad5CMV (n = 4 mice) vectors, and probed for EGFP protein expression normalized to either VE-cadherin or β-tubulin (Figures 4A–D).