The principles of polyfunctionality also seem to apply to other T-cells populations as evidence suggests that there is a shift in the distribution pattern of cytokine expression of CD3+ T-cells from exclusively IL-2 or TNF-α expression seen in healthy controls towards an increased frequency of IFN-γ/IL-2 or IFN-γ/TNF-α coexpression in TB indicating an obviously disease-associated shift from early memory cellular subpopulations in controls to activated and type 1 biased T-cells in TB patients [29]. This evidence concerns the gene IFNG and tuberculosis.