ESR1 and neoplasm: Additional analyses indicated also that the triple stimulation of TNFα + Estrogen + EGF was more effective in inducing spreading and cell remodeling than dual stimulations by Estrogen +TNFα or Estrogen + EGF (Figure S1 in Supplementary Material available online at http://dx.doi.org/10.1155/2013/720536) (the dual stimulations focused on combinations including estrogen because it is the most relevant factor to the luminal tumor cells we were using, characterized by ER expression).