TWIST1 and ovarian carcinoma: Consistent with earlier findings in an ovarian cancer model [36], there was a significant switch toward more genes marked by H3K27me3 in MS cells (3,607) compared to monolayer (2,411; Figure S9B in Additional file 10), but the majority of these genes were already transcriptionally silenced in monolayer culture in response to the overexpression of Twist1.