MBP and neuromyelitis optica: Since EAE and NMO/EAE in Lewis rats are TH1-driven diseases with IFN-γ as lead cytokine, we next determined the IFN-γ expression of the re-isolated MBP-, S100β-, and MOG-specific T cells by RT-PCR, and observed that MBP-specific T cells had significantly higher relative IFN-γ mRNA expression levels than their S100β- or MOG-specific counterparts (Figure 8).