To further elucidate whether a functional cross talk exists between the two pathways in NSCLC, we examined the impact of CHIR99021, a Wnt signaling agonist, or Notch3-shRNA, or the combined application of CHIR99021 and Notch3-shRNA on cell proliferation and apoptosis. The gene discussed is NOTCH3; the disease is non-small cell lung carcinoma.