While HK1 levels were not altered (Fig 4A), protein levels of PFK1-P, which is highly expressed in cerebellum [52], were increased in Npc1-/- cerebellum at 5 weeks but not at 3 weeks of age (Fig 4B), further pointing to an increase in glycolysis in cerebellum at the early symptomatic stage of NPC disease. The gene discussed is NPC1; the disease is nasopharyngeal carcinoma.