While HK1 levels were not altered (Fig 4A), protein levels of PFK1-P, which is highly expressed in cerebellum [52], were increased in Npc1-/- cerebellum at 5 weeks but not at 3 weeks of age (Fig 4B), further pointing to an increase in glycolysis in cerebellum at the early symptomatic stage of NPC disease. This evidence concerns the gene HK1 and nasopharyngeal carcinoma.