Together, these findings demonstrated that alterations in cerebellar glucose metabolism (upregulation of Eno1 and Pcx mRNA, higher PKM2 protein, decreased Pdha, Me1 and Me2 mRNA levels, increased lactate and decreased acetate/acetyl-CoA levels) developed early in NPC disease, and could already be detected in 3-week old, pre-symptomatic Npc1-/- mice. This evidence concerns the gene NPC1 and nasopharyngeal carcinoma.