The results demonstrated that high expression of ferroportin was observed in normal human liver cell lines L02 and HL7702, while weak positive expression and even negative expression of ferroportin was observed in HCC cell lines FOCUS, MHCC-97H, HepG2 and SMMC-7721 (Figure 2A,B). The gene discussed is SLC40A1; the disease is hepatocellular carcinoma.