To determine whether detection of B. burgdorferi will be affected by the presence of higher levels of bacteremia and parasitemia due to A. phagocytophilum and B. microti, respectively, we mixed genomic DNA of all three pathogens such that the copy number of BmTPK and APH1387 was 100-fold higher than that of the recA copies of B. burgdorferi. This evidence concerns the gene RAD51 and bacterial infectious disease with sepsis.