Then, after 60 days in culture, all control clones were at fourth passage (p4) and three of them, named C2, C7 and C10, presented altered morphology (spindle shape) (Figure 6A) as that presented by 4C3+, 4C11+, Tm1 and Tm5 metastatic melanoma cell lines established by our laboratory using the same anchorage substrate impediment approach [16]. This evidence concerns the gene TPM3 and metastatic melanoma.