Considering that mRNA levels of Notch receptors are frequently not representative of the protein levels of N1-ICD, activated Notch1 pathway status in this cohort was inferred from expression levels of a Notch-dependent gene signature, built up by selecting published Notch1 targets, for which Notch responsiveness and/or direct promoter binding as well as their expression in breast cancer was demonstrated (Notch direct target gene signature, NDT, supplementary Table S2). The gene discussed is NOTCH1; the disease is breast cancer.