In a recently published study, it has been shown that Akt-mediated Twist1 phosphorylation promotes epithelial-mesenchymal transition and breast cancer metastasis by modulating its transcriptional target TGF-β2; this leads to enhanced TGF-β receptor signaling, which in turn maintains hyperactive PI3K/Akt signaling (45). This evidence concerns the gene TWIST1 and breast cancer.