Mice with similar sizes of ovarian tumors were selected to receive treatment with NKG2D-Fc or NKG2D-Fc-RO and OVA-specific OT-1 CD8+ T cells injected intraperitoneally, as outlined in the treatment schedule depicted in Figure 5A. We found that female TgMISIIR-TAg transgenic mice treated with NKG2D-Fc-RO had significantly reduced tumor mass after 30 days compared to those treated with NKG2D-Fc (Figure 5B and C). This evidence concerns the gene KLRK1 and ovarian neoplasm.