In order to determine if treatment of the spontaneous ovarian tumor-bearing mice with the chimeric NKG2D-Fc-RO protein in conjunction with adoptive transfer of OVA-specific CD8+ T cell can lead to the expansion of OVA-specific CD8+ T cells in the ovarian tumor loci, we characterized the presence of OVA-specific CD8+ T cell among the TILs derived from the ovarian tumors using OVA CTL peptide loaded H-2Kb tetramer labeling. This evidence concerns the gene KLRK1 and ovarian neoplasm.