Comparison of the SFRP2, WIF-1, DKK3 and SOX17 promoter methylation levels between nine CRC cell lines and eighteen normal colon mucosa samples revealed significantly elevated methylation levels in CRC cells for all four genes (p = 0.00001, p = 3.1*10-5, p = 3.1*10-5 and p = 0.001 for SFRP2, WIF-1, DKK3 and SOX17, respectively). This evidence concerns the gene DKK3 and colorectal carcinoma.