SSCP followed by direct sequencing of all 16 exons of NBS1 revealed eight missense NBS1 mutations in eight of the 82 (11.0%) cases of primary liver cancer (Table 2, Figure 1A-B), distributed among exons 2, 3, 7, 9, 10,11 and 12 (Figure 2). The gene discussed is NBN; the disease is liver cancer.