TNF and neoplasm: BMDM of control and orlistat-administered groups were incubated for 24 h in medium alone or containing LPS+IFN-γ followed by estimation of M1 macrophage markers: NO (Figure 6a), level of IL-1, IL-6 & TNF-α in the culture supernatant (Figure 6b), phagocytosis (Figure 6c), expression of ROS (Figure 6 d,e), tumor cytotoxicity (Figure 6f,g) and pattern of cell surface-associated functional proteins: CD11c & TLR-2 (Figure 6h).