As the expression level of phosphoenolpyruvate carboxykinase (PEPCK), a critical enzyme that catalyzes the conversion of oxaloacetate to phosphoenol pyruvate, is negatively correlated with the level of blood glucose in acute hyperglycemia while up-regulated by chronic hyperglycemia in type 2 diabetes, [20], [21] we also determined the effect of HCD-HG treatment on the expression of PEPCK by real-time PCR. This evidence concerns the gene PCK2 and type 2 diabetes mellitus.