In the present study, siRNA duplexes targeting human EGFR and bFGFR were designed, synthesized and used to knock down EGFR and/or FGFR gene expression, in order to aid in the elucidation of the roles of EGF-EGFR and FGF-FGFR autocrine pathways in the differentiation of U251 glioblastoma cells. This evidence concerns the gene EGF and glioblastoma.