Eu3+-labeled hALR was used to compete with the pure hALR protein as an antigen in the competitive inhibition, and an anti-hALR monoclonal hybridoma cell line was used to produce anti-hALR monoclonal antibody; these processes established a direct competitive method for measuring serum hALR to aid the understanding of serum hALR concentration and its significance in various liver diseases. This evidence concerns the gene KMT2C and liver disorder.