Furthermore, Mulé and colleagues have recently performed a metagene analysis on human (Stage IV, non-locoregional) melanoma metastases and identified a 12-chemokine gene signature (i.e., CCL2, CCL3, CCL4, CCL5, CCL8, CCL18, CCL19, CCL21, CXCL9, CXCL10, CXCL11, CXCL13) correlating with the presence of TLO (containing CD20+ B cell follicles with prominent areas of CD4+ and CD8+ T cells, but not FoxP3+ Treg cells), with better overall survival noted in the TLO+ subset of patients (41). This evidence concerns the gene CCL21 and melanoma.