TRIO and infection: EGL-30(Gαq) activates a Rho GEF TRIO – RHOA - RAF signaling pathway to trigger the Dar phenotype in response to infection and activation of downstream components of this pathway in the rectal epithelium; using cell-specific overexpression of constitutively active forms of RHO-1(G14V) (RhoA) (RE::RHO-1*) or LIN-45(S312A,S453A) (Raf) (RE::LIN-45*), also results in the Dar phenotype in the absence of infection.