ALDH1A3 and sarcoma: Thus, we validated both the expression and methylation of ALDH1A3. Using pyrosequencing, the mean level of methylation in the first intron of ALDH1A3 in six non-neoplastic fat samples was found to be 23.5% compared to 73.6% in nine MLS samples from the sarcoma collection (Additional file 2: Figure S9d,e).