EF+/DTF + breast cancers were less likely to be ER negative (7%), PR negative (11.76%), Grade 3 (7.61%) and p53 mutant (8.96%) than EF-/DTF- breast cancers that were ER negative (24.51%), PR negative (31.82%), Grade 3 (40.48%) and p53 mutant (32.88%) (see Additional file 11). The gene discussed is TP53; the disease is breast cancer.