EGFR and neoplasm: To validate the pattern of genetic amplifications of EGFR, FISH assays with LSI EGFR SpectrumOrange/CEP 7 SpectrumGreen Probe kit were performed in the 26 MPNST samples from TMUCIH (the matching fresh-frozen MPNST tissues were used in the aCGH analysis ) (Figure 2B), which confirmed EGFR gene amplification in large fragment pattern in tumor cells (Figure 2C).