To analyze if the changes observed in gene expression were translated into changes at protein level, we performed a Western blot experiment of the two most differentially expressed genes SCN2B and KCNJ5. We did not found statistically significant differences between the DCM group and the CNT group in the SCN2B protein levels (78 ± 19 vs. 100 ± 31, respectively), and the same results were obtained comparing these two groups in the KCNJ5 protein levels (128 ± 34 vs. 100 ± 27, respectively) (data not shown). This evidence concerns the gene SCN2B and familial dilated cardiomyopathy.