The NOL4 gene showed no methylation (0/16) in normal salivary rinses and two out of 14 (14%) mucosal samples were minimally methylated between 1 and 3% methylation values, whereas the methylation rate was 91% (30/33) on the promoter region of the NOL4 gene in HNSCC tumor samples showing high methylation values are 79% (26/33) of the patients between 10 and 100%. This evidence concerns the gene NOL4 and head and neck squamous cell carcinoma.