In a second part, as Aβ42 did not modify autophagy in tri-cultures contrary to LPS and in order to better understand cytokine involvement in autophagy regulation in AD, we tested the impact of a cytokine cocktail (IL-1β, TNF-α, and IL-6) and each cytokine alone in tri-cultures exposed to Aβ42. The gene discussed is TNF; the disease is Alzheimer disease.