To test whether FLOT1 is the direct functional mediator of the miR-124 induced inhibition of breast cancer cell proliferation and migration, we co-transfected miR-124 mimics along with wt/mut 3′-UTR-FLOT1 plasmid which FLOT1 cDNA contained wild type or mutant 3′ UTR into breast cancer cells. The gene discussed is FLOT1; the disease is breast carcinoma.