TWIST1 and breast cancer: This hypothesis was confirmed by Q-PCR which showed a significantly increased expression of twist1, (fold change 4,99 in KBr1 and 2,16 in KBr2) and snail1 (fold change 7,55 in KBR1 and 4,46 in KBr2) in both breast cancer samples, showing higher migratory activity after co-culture with ASCs (Figure 2B).