In a glioblastoma study, Notch blockade by GBI appeared to deplete stem-like cancer cells via reduced proliferation and increased apoptosis associated with decreased AKT and signal transducer and activator of transcription 3 phosphorylation, which was consistent with reduced expression of the CSC markers CD133, Nestin, Bmi1, and olig2 [102]. Here, NES is linked to cancer.