To address whether genetic alterations contribute to the dysregulation of DLGAP5 in HCCs, we used a custom-designed Multiplex AccuCopy Kit (Genesky Biotechnologies Inc., Shanghai, China) to detect DNA copy number changes in HCC tissues compared with non-HCC tissues based on the previously described multiplex fluorescence competitive PCR principle. Here, DLGAP5 is linked to hepatocellular carcinoma.