The expression of a number of genes was strongly up-regulated in the ASC lines compared to the AC lines (Figure 5) including: MAGEA3, MAGEA6, CSTA, TFPI2, KRT5, TWIST1 and PTHLH. Of these, only the MAGEA3/A6 expression was entirely restricted to lung cancer cells (>30X higher in ASC than AC) and not seen at all in the tumor stroma or normal lung samples. This evidence concerns the gene TWIST1 and neoplasm.