Undiluted (1 : 0) and 10- and 100-fold diluted CFA adjuvant was used in NOD mouse inoculation studies in combination with the recombinant virus rVV-CTB::GAD to assess the efficacy of the multifunctional vaccine in autoantigen-dependent enhancement of immune suppression of T1D symptoms of hyperglycemia and insulitis. Here, GAD1 is linked to Hyperglycemia.