PHGDH and breast cancer: Expression of PHGDHR cDNA restored the expression of PHGDH in MDA-MB-468 cells when endogenous PHGDH expression was depleted by Doxycycline treatment(Fig. 3A) and rescued the cell proliferation phenotype medicated by PHGDH knockdown (Fig. 3B), which indicates that the inhibitory effects of PHGDH knockdown on the proliferation of breast cancer cells is on-target.