Recent studies have identified other potential biomarkers for early detection of HCC, including the circulating AFP isoform AFP-L3, des-gamma-carboxy prothrombin (DCP), and Golgi protein-73 (GP73), although these biomarkers have been used in the clinic [4–6], the practical value of these markers has yet to be fully evaluated. This evidence concerns the gene ACE and hepatocellular carcinoma.