Lentiviral silencing ANO1 was used in scc-25 cell line to study the cell migration and cell detachment.Results: Immunohistochemical staining revealed that ANO1 was expressed in a large majority (132 out of 160, 82.5%) of OSCC specimens and that the rate of ANO1 expression in OSCC was significantly higher than that of normal tissue (P<0.05); The rate of ANO1 expression was higher in metastatic tumors than in non-metastatic tumors, and the difference was significant (P<0.05). The gene discussed is ANO1; the disease is metastatic neoplasm.