In order to determine the effect of IL-4 or IL-10 therapy on TNBS-induced murine colitis, colonic levels of IFN-γ protein were assessed using ELISA on all mice on day 7 and the results showed that TNBS-treated mice had elevated levels of IFN-γ (1043.804 pg/ml), whereas IL-4 or IL-10 therapy significantly reduced levels of IFN-γ protein (593.116 pg/ml and 421.204 pg/ml, respectively). The gene discussed is IFNG; the disease is colitis.