It should be noted that the human Ad4 vaccine vectors expressing HIV-1 Env described in this paper do not carry out productive infections in cells of nonhuman origin but, similar to defective Ad5 vectors, they will infect cells from multiple species including mice, rabbits, and many species of NHPs, and these cells will effectively support transcription and translation of both Ad viral proteins and foreign transgenes. Here, PSEN2 is linked to infection.