Western blot analysis (Figure 6C) reaffirmed that peritoneal macrophages from WT but not Nlrp3−/− mice secreted mature IL-1β to cell supernatants after infection with C. rodentium, and as well, that administration of exogenous IL-1β induced maturation of the cytokine in uninfected WT macrophages. Here, NLRP3 is linked to infection.