BMI1 and cancer: To reveal the functional relevance of miR-30e* expression, we examined the Bmi1 expression in the 6 gastrointestinal cancer cell lines by Western blotting (Figure 3A), and analyzed the relationship between miR-30e* and Bmi1 expression in high Bmi1 expressing cancer cell lines (AGS and HCT116) transfected with miR-30e* mimics, and low Bmi1 expressing cancer cell lines (NUGC4 and COLO201) transfected with miR-30e* inhibitors.