We also found that down-regulation of miR-126 could promote AKT and ERK1/2 activation by targeting IRS-1.Taken together, our results imply that up-regulation of miR-126 could down-regulate IRS-1, inhibit CRC cells proliferation, migration, invasion, and induce cell cycle arrest, but not in apoptosis of CRC cells, partly through AKT and ERK1/2 signaling pathways. This evidence concerns the gene MAPK3 and colorectal carcinoma.