We also tested the potential role of PI3K signaling in our zebrafish chordoma model using the PI3K inhibitor LY294002 and the PTEN inhibitor bpV(HOpic), but did not observe any inhibitory or activating effects on tumor growth, possibly due to PI3K-independent activation of mTOR by the RAS oncogene (Shaw and Cantley, 2006). Here, MTOR is linked to chordoma.